(a) General Comments. Keep the equipment in good working condition. Don't
use anything (any instrument) unless you have been instructed in its
proper use. Report any malfunction immediately. Rinse out all centrifuge
rotors after use, in particular if anything spills.
Please do not waste supplies—use only what you need. If the supply is
running low, please notify the instructor before it is completely exhausted.
Occasionally, it is necessary to borrow a reagent or equipment from another
lab; notify the instructor.
(b) Micropipettors. Most of the experiments you will conduct in the laboratory
will depend on your ability to accurately measure volumes of solutions
using micropipettors. The accuracy of your pipetting can only be as accurate
as your pipettor, and several steps should be taken to ensure that your
pipettes are accurate and maintained in good working order. Then they
should checked for accuracy following the instructions given by the
instructor. If they need to be recalibrated, do so.
There are 2 different types of pipettors, Rainin pipetmen and Oxford
benchmates. Since the pipettors will use different pipette tips, make sure
that the pipette tip you are using is designed for your pipettor.
(c) Using a pH Meter. Biological functions are very sensitive to changes in pH
and hence, buffers are used to stabilize the pH. A pH meter is an instrument
that measures the potential difference between a reference electrode
and a glass electrode, often combined into one combination electrode. The
reference electrode is often AgCl2. An accurate pH reading depends on
standardization, the degree of static charge, and the temperature of the
solution.
(d) Autoclave Operating Procedures. Place all material to be autoclaved on an
autoclavable tray. All items should have indicator tape. Separate liquids
from solids and autoclave separately. Make sure the lids on all bottles are
loose.
Make sure the chamber pressure is at zero before opening the door.
use anything (any instrument) unless you have been instructed in its
proper use. Report any malfunction immediately. Rinse out all centrifuge
rotors after use, in particular if anything spills.
Please do not waste supplies—use only what you need. If the supply is
running low, please notify the instructor before it is completely exhausted.
Occasionally, it is necessary to borrow a reagent or equipment from another
lab; notify the instructor.
(b) Micropipettors. Most of the experiments you will conduct in the laboratory
will depend on your ability to accurately measure volumes of solutions
using micropipettors. The accuracy of your pipetting can only be as accurate
as your pipettor, and several steps should be taken to ensure that your
pipettes are accurate and maintained in good working order. Then they
should checked for accuracy following the instructions given by the
instructor. If they need to be recalibrated, do so.
There are 2 different types of pipettors, Rainin pipetmen and Oxford
benchmates. Since the pipettors will use different pipette tips, make sure
that the pipette tip you are using is designed for your pipettor.
(c) Using a pH Meter. Biological functions are very sensitive to changes in pH
and hence, buffers are used to stabilize the pH. A pH meter is an instrument
that measures the potential difference between a reference electrode
and a glass electrode, often combined into one combination electrode. The
reference electrode is often AgCl2. An accurate pH reading depends on
standardization, the degree of static charge, and the temperature of the
solution.
(d) Autoclave Operating Procedures. Place all material to be autoclaved on an
autoclavable tray. All items should have indicator tape. Separate liquids
from solids and autoclave separately. Make sure the lids on all bottles are
loose.
Make sure the chamber pressure is at zero before opening the door.
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