Disposal of Buffers and Chemicals Laboratory Methods

(i) Any uncontaminated, solidified agar or agarose should be discarded in the
trash, not in the sink, and the bottles rinsed well.
(ii) Any media that becomes contaminated should be promptly autoclaved
before discarding it. Petri dishes and other biological waste should be discarded

in biohazard containers, which will be autoclaved prior to disposal.

(iii) Organic reagents, e.g., phenol, should be used in a fume hood and all
organic waste should be disposed of in a labeled container, not in the
trash or the sink.
(iv) Ethidium bromide is a mutagenic substance that should be treated before
disposal and handled only with gloves. Ethidium bromide should be
disposed off in a labeled container.